Project

Part:BBa_K3113210

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-10-13)


pCAG_CD63_Asn180_6xHis_Hibit_L7Ae

This plasmid encodes for an engineered version of CD63 fused to the HiBiT peptide and L7Ae. The 6xHis tag inserted into the large extracellular loop at Asn180 of CD63 is used to facilitate purification of successfully engineered exosomes. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection. The HiBit peptide is used for detection via luminescence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None